Ratiometric imaging of calcium during ischemia-reperfusion injury in isolated mouse hearts using Fura-2

BACKGROUND: We present an easily implementable method for measuring Fura-2 fluorescence from isolated mouse hearts using a commercially available switching light source and CCD camera. After calibration, it provides a good estimate of intracellular [Ca(2+)] with both high spatial and temporal resolu...

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Hauptverfasser: Venkataraman, Raghav, Holcomb, Mark R, Harder, Rene, Knollmann, Björn C, Baudenbacher, Franz
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2012
Schlagworte:
Research
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3466138/
https://ncbi.nlm.nih.gov/pubmed/22812644
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-925X-11-39
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