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Ratiometric imaging of calcium during ischemia-reperfusion injury in isolated mouse hearts using Fura-2
BACKGROUND: We present an easily implementable method for measuring Fura-2 fluorescence from isolated mouse hearts using a commercially available switching light source and CCD camera. After calibration, it provides a good estimate of intracellular [Ca(2+)] with both high spatial and temporal resolu...
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| Autors principals: | , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
BioMed Central
2012
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3466138/ https://ncbi.nlm.nih.gov/pubmed/22812644 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-925X-11-39 |
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