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Computational design and characterization of a temperature-sensitive plasmid replicon for gram positive thermophiles
BACKGROUND: Temperature-sensitive (Ts) plasmids are useful tools for genetic engineering, but there are currently none compatible with the gram positive, thermophilic, obligate anaerobe, Clostridium thermocellum. Traditional mutagenesis techniques yield Ts mutants at a low frequency, and therefore r...
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| Главные авторы: | , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
BioMed Central
2012
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3464808/ https://ncbi.nlm.nih.gov/pubmed/22578246 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1754-1611-6-5 |
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