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Isolation, characterization, and utilization of a temperature-sensitive allele of a Pseudomonas replicon
In order to facilitate genetic study of the opportunistic bacterial pathogen Pseudomonas aeruginosa, we isolated a conditional, temperature-sensitive plasmid origin of replication. We mutagenized the popular Pseudomonas stabilizing fragment from pRO1610 in vitro using the Taq thermostable DNA polyme...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2782952/ https://ncbi.nlm.nih.gov/pubmed/19615413 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2009.07.002 |
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