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Rational Design of a Plasmid Origin That Replicates Efficiently in Both Gram-Positive and Gram-Negative Bacteria
BACKGROUND: Most plasmids replicate only within a particular genus or family. METHODOLOGY/PRINCIPAL FINDINGS: Here we describe an engineered high copy number expression vector, pBAV1K-T5, that produces varying quantities of active reporter proteins in Escherichia coli, Acinetobacter baylyi ADP1, Agr...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2010
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2951906/ https://ncbi.nlm.nih.gov/pubmed/20949038 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0013244 |
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