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Identification and characterization of a proton pump on lysosomes by fluorescein-isothiocyanate-dextran fluorescence.

Fluorescein isothiocyanate-conjugated dextran was introduced preferentially into hepatic lysosomes by intraperitoneal injection into rats. The pH in isolated lysosomes, measured by fluorescein fluorescence, was approximately 5 and gradually increased in KCl (to 7.0) at 25 degrees C. In the presence...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Ohkuma, S, Moriyama, Y, Takano, T
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1982
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Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346285/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6178109/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.9.2758
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