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Identification and characterization of a proton pump on lysosomes by fluorescein-isothiocyanate-dextran fluorescence.
Fluorescein isothiocyanate-conjugated dextran was introduced preferentially into hepatic lysosomes by intraperitoneal injection into rats. The pH in isolated lysosomes, measured by fluorescein fluorescence, was approximately 5 and gradually increased in KCl (to 7.0) at 25 degrees C. In the presence...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1982
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346285/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6178109/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.9.2758 |
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