Reconstitution of the lysosomal proton pump.

Lysosomal membrane proteins solubilized with octyl beta-D-glucopyranoside were reconstituted into proteoliposomes using acetone/ether-washed phospholipids from Escherichia coli. Assays of the quenching of acridine orange fluorescence showed that addition of both ATP and valinomycin to K+-loaded prot...

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Bibliografski detalji
Glavni autori: D'Souza, M P, Ambudkar, S V, August, J T, Maloney, P C
Format: Artigo
Jezik:Inglês
Izdano: 1987
Teme:
Research Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC299212/
https://ncbi.nlm.nih.gov/pubmed/2890158
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