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Reconstitution of the lysosomal proton pump.

Lysosomal membrane proteins solubilized with octyl beta-D-glucopyranoside were reconstituted into proteoliposomes using acetone/ether-washed phospholipids from Escherichia coli. Assays of the quenching of acridine orange fluorescence showed that addition of both ATP and valinomycin to K+-loaded prot...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: D'Souza, M P, Ambudkar, S V, August, J T, Maloney, P C
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1987
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC299212/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2890158/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.20.6980
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