Affinity chromatography purification of cytochrome c binding enzymes.
An efficient affinity chromatography procedure for the isolation of mitochondrial cytochrome c oxidase and reductase is described. Saccharomyces cerevisiae cytochrome c was used as a ligand, bound to a thiol-Sepharose 4B gel through cysteine-107. In this way, the site of interaction of cytochrome c...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1982
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346215/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6283525/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.8.2447 |
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