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Affinity chromatography with an immobilized RNA enzyme.
M1 RNA, the catalytic subunit of Escherichia coli RNase P, has been covalently linked at its 3' terminus to agarose beads. Unlike M1 RNA, which is active in solution in the absence of the protein component (C5) of RNase P, the RNA linked to the beads is active only in the presence of C5 protein...
Guardat en:
| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1986
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC386405/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3526344/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.16.5904 |
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