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Purification of Pseudomonas aeruginosa exotoxin by affinity chromatography.

Pseudomonas aeruginosa exotoxin A was purified by affinity chromatography from culture supernatants by elution of toxin from antitoxin immunoglobulin G-Sepharose 4B with 3 M NaSCN. The purity, toxicity, and enzymatic activity of exotoxin obtained were comparable to those of toxin purified by previou...

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Détails bibliographiques
Publié dans:Infect Immun
Auteurs principaux: Taylor, N S, Pollack, M
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1978
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC414049/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/415008/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.19.1.66-70.1978
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