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Solubilization and purification of the alpha 1-adrenergic receptor using a novel affinity resin.
The highly selective alpha 1-adrenergic receptor antagonist prazosin was used to identify binding sites having alpha-adrenergic specificity in rat hepatic plasma membranes. Solubilization of the membrane-bound receptors was achieved by incubation with the nonionic detergent digitonin, and binding ac...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1982
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346155/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6285370/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.7.2186 |
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