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Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae

The cystic fibrosis transmembrane conductance regulator (CFTR) is a chloride channel, that when mutated, can give rise to cystic fibrosis in humans.There is therefore considerable interest in this protein, but efforts to study its structure and activity have been hampered by the difficulty of expres...

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Detalhes bibliográficos
Main Authors: O'Ryan, Liam, Rimington, Tracy, Cant, Natasha, Ford, Robert C.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3460588/
https://ncbi.nlm.nih.gov/pubmed/22433465
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3860
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