Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates

Proteins of the Rho family of small GTPases are central regulators of the cytoskeleton, and control a large variety of cellular processes, including cell migration, gene expression, cell cycle progression and cell adhesion (1). Rho proteins are molecular switches that are active in GTP-bound and ina...

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Autori principali: Waheed, Faiza, Speight, Pamela, Dan, Qinghong, Garcia-Mata, Rafael, Szaszi, Katalin
Natura: Artigo
Lingua:Inglês
Pubblicazione: MyJove Corporation 2012
Soggetti:
Molecular Biology
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3460580/
https://ncbi.nlm.nih.gov/pubmed/22491204
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3932
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