Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro.
Short single-stranded gaps can be constructed by limited exonuclease action at single-stranded breaks (nicks) placed at predetermined sites on closed circular DNA molecules. As efficient primer-templates for DNA polymerase, single-stranded gaps can be repaired in vitro to regenerate an intact DNA du...
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| Vydáno v: | Proc Natl Acad Sci U S A |
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| Hlavní autoři: | , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1982
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346020/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7041125/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.5.1588 |
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