Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro.

Short single-stranded gaps can be constructed by limited exonuclease action at single-stranded breaks (nicks) placed at predetermined sites on closed circular DNA molecules. As efficient primer-templates for DNA polymerase, single-stranded gaps can be repaired in vitro to regenerate an intact DNA du...

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Bibliographic Details

Published in:	Proc Natl Acad Sci U S A
Main Authors:	Shortle, D, Grisafi, P, Benkovic, S J, Botstein, D
Format:	Artigo
Language:	Inglês
Published:	National Academy of Sciences 1982
Subjects:	Research Article
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346020/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7041125/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.5.1588
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