Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.
DNA from simian virus 40 (SV40) was prepared for local mutagenesis by nicking the molecule at a specific site with a restriction endonuclease that recognizes one site in SV40 DNA and then extending the nick enzymatically to expose a short, single-stranded segment of DNA. The "gapped" DNA was treated...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
1978
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392513/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/209457/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.5.2170 |
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