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Mechanisms of DNA uptake by mammalian cells: fate of exogenously added DNA monitored by the use of fluorescent dyes.
Coprecipitation of DNA with calcium phosphate is a commonly used method of gene transfer in mammalian cells. We have found that DNA forms a tight complex with Ca Pi and that DNA in this complex is resistant to nucleases present in serum or added externally. Under optimal conditions, virtually all of...
Shranjeno v:
| izdano v: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1982
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC345755/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6952193/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.2.422 |
| Oznake: |
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