Mechanisms of DNA uptake by mammalian cells: fate of exogenously added DNA monitored by the use of fluorescent dyes.

Coprecipitation of DNA with calcium phosphate is a commonly used method of gene transfer in mammalian cells. We have found that DNA forms a tight complex with Ca Pi and that DNA in this complex is resistant to nucleases present in serum or added externally. Under optimal conditions, virtually all of...

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Bibliografiske detaljer

Udgivet i:	Proc Natl Acad Sci U S A
Principais autores:	Loyter, A, Scangos, G A, Ruddle, F H
Format:	Artigo
Sprog:	Inglês
Udgivet:	National Academy of Sciences 1982
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC345755/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6952193/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.2.422
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