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Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant
A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluc...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3457482/ https://ncbi.nlm.nih.gov/pubmed/22798371 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01794-12 |
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