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Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant

A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluc...

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書誌詳細
主要な著者: Schreier, Harold J., Dejtisakdi, Wipawee, Escalante, Jose Ortega, Brailo, Marina
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology 2012
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3457482/
https://ncbi.nlm.nih.gov/pubmed/22798371
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01794-12
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