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Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant

A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluc...

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Main Authors: Schreier, Harold J., Dejtisakdi, Wipawee, Escalante, Jose Ortega, Brailo, Marina
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2012
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3457482/
https://ncbi.nlm.nih.gov/pubmed/22798371
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01794-12
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