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The “Spanning Protocol”: A new DNA extraction method for efficient single-cell genetic diagnosis
Purpose: We evaluated methods of preparation of DNA from single cells for amplification and preimplantation genetic diagnosis (PGD), including our “spanning protocol.” Methods: Dystrophin gene exons 45 and 51 were amplified by nested polymerase chain reaction (PCR) from a single lymphocyte or blasto...
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| Hoofdauteurs: | , , , , , , , |
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| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
Kluwer Academic Publishers-Plenum Publishers
2005
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3455149/ https://ncbi.nlm.nih.gov/pubmed/16331538 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10815-005-7482-x |
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