The “Spanning Protocol”: A new DNA extraction method for efficient single-cell genetic diagnosis

Purpose: We evaluated methods of preparation of DNA from single cells for amplification and preimplantation genetic diagnosis (PGD), including our “spanning protocol.” Methods: Dystrophin gene exons 45 and 51 were amplified by nested polymerase chain reaction (PCR) from a single lymphocyte or blasto...

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Main Authors: Tsuchiya, Shinichi, Sueoka, Kou, Matsuda, Noriko, Tanigaki, Reiko, Asada, Hironori, Hashiba, Tsuyoshi, Kato, Shinya, Yoshimura, Yasunori
Formato: Artigo
Idioma:Inglês
Publicado: Kluwer Academic Publishers-Plenum Publishers 2005
Assuntos:
Genetics
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3455149/
https://ncbi.nlm.nih.gov/pubmed/16331538
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10815-005-7482-x
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