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Homologous recombination between plasmids in mammalian cells can be enhanced by treatment of input DNA.
We have used the eukaryotic-prokaryotic shuttle vector pSV2Neo to demonstrate that cultured mammalian somatic cells have the enzymatic machinery to mediate homologous recombination and that the frequency of this recombination can be enhanced by pretreatment of the input DNA. Two nonoverlapping delet...
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| 出版年: | Proc Natl Acad Sci U S A |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
National Academy of Sciences
1984
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC345239/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6328502/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.10.3153 |
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