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Homologous recombination between plasmids in mammalian cells can be enhanced by treatment of input DNA.

We have used the eukaryotic-prokaryotic shuttle vector pSV2Neo to demonstrate that cultured mammalian somatic cells have the enzymatic machinery to mediate homologous recombination and that the frequency of this recombination can be enhanced by pretreatment of the input DNA. Two nonoverlapping delet...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Kucherlapati, R S, Eves, E M, Song, K Y, Morse, B S, Smithies, O
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1984
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC345239/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6328502/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.10.3153
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