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Production of recombinant granulocyte colony-stimulating factor by knocking into the active immunoglobulin heavy chain gene locus in the hybridoma cell line

The hybridoma cell line KM50 originally produces a monoclonal antibody at a concentration of ∼40 mg ml(-1) in ascites. To investigate the possibility to apply this expression system to the production of useful proteins, the cDNA encoding human granulocyte colony-stimulating factor was inserted by ho...

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Autors principals: Kuwana, Yoshihisa, Funayama, Kikuko, Miyaji, Hiromasa, Hasegawa, Mamoru, Yoshida, Hajime, Itoh, Seiga
Format: Artigo
Idioma:Inglês
Publicat: Kluwer Academic Publishers 2001
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3449790/
https://ncbi.nlm.nih.gov/pubmed/19002916
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1020585320775
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