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Production of recombinant granulocyte colony-stimulating factor by knocking into the active immunoglobulin heavy chain gene locus in the hybridoma cell line
The hybridoma cell line KM50 originally produces a monoclonal antibody at a concentration of ∼40 mg ml(-1) in ascites. To investigate the possibility to apply this expression system to the production of useful proteins, the cDNA encoding human granulocyte colony-stimulating factor was inserted by ho...
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| Главные авторы: | , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Kluwer Academic Publishers
2001
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449790/ https://ncbi.nlm.nih.gov/pubmed/19002916 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1020585320775 |
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