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Production of recombinant granulocyte colony-stimulating factor by knocking into the active immunoglobulin heavy chain gene locus in the hybridoma cell line
The hybridoma cell line KM50 originally produces a monoclonal antibody at a concentration of ∼40 mg ml(-1) in ascites. To investigate the possibility to apply this expression system to the production of useful proteins, the cDNA encoding human granulocyte colony-stimulating factor was inserted by ho...
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| Hlavní autoři: | , , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Kluwer Academic Publishers
2001
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449790/ https://ncbi.nlm.nih.gov/pubmed/19002916 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1020585320775 |
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