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Recombinant protein production using the Semliki Forest Virus expression system
We report here the successful scale up of transient recombinant protein expression to litre scale using Semliki Forest Virus System. The expression of bacterial β-galactosidase was initially compared in BHK and CHO cells and the conditions for optimal infection of BHK cells were identified. 10% FCS...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Kluwer Academic Publishers
1997
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449614/ https://ncbi.nlm.nih.gov/pubmed/22358598 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1007974121182 |
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