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Recombinant protein production using the Semliki Forest Virus expression system

We report here the successful scale up of transient recombinant protein expression to litre scale using Semliki Forest Virus System. The expression of bacterial β-galactosidase was initially compared in BHK and CHO cells and the conditions for optimal infection of BHK cells were identified. 10% FCS...

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Detalhes bibliográficos
Main Authors: Blasey, H.D., Lundström, K., Tate, S., Bernard, A.R.
Formato: Artigo
Idioma:Inglês
Publicado em: Kluwer Academic Publishers 1997
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3449614/
https://ncbi.nlm.nih.gov/pubmed/22358598
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1007974121182
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