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Recombinant protein production using the Semliki Forest Virus expression system

We report here the successful scale up of transient recombinant protein expression to litre scale using Semliki Forest Virus System. The expression of bacterial β-galactosidase was initially compared in BHK and CHO cells and the conditions for optimal infection of BHK cells were identified. 10% FCS...

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Sonraí Bibleagrafaíochta
Main Authors: Blasey, H.D., Lundström, K., Tate, S., Bernard, A.R.
Formáid: Artigo
Teanga:Inglês
Foilsithe: Kluwer Academic Publishers 1997
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3449614/
https://ncbi.nlm.nih.gov/pubmed/22358598
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1007974121182
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