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In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon.

A mini-Mu bacteriophage containing a high copy number plasmid replicon was constructed to clone genes in vivo. A chloramphenicol resistance gene for independent selection and the lacZYA operon to form gene fusions were also incorporated into this phage. This mini-Mu element can transpose at a high f...

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Bibliografiske detaljer
Main Authors: Groisman, E A, Castilho, B A, Casadaban, M J
Format: Artigo
Sprog:Inglês
Udgivet: 1984
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC344860/
https://ncbi.nlm.nih.gov/pubmed/6324195
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