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In vivo cloning of Pseudomonas aeruginosa genes with mini-D3112 transposable bacteriophage.

The transposition properties of the Pseudomonas aeruginosa mutator bacteriophage D3112 were exploited to develop an in vivo cloning system. Mini-D replicon derivatives of D3112 were constructed by incorporating broad host range plasmid replicons between short terminal D3112 sequences. These elements...

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Main Authors: Darzins, A, Casadaban, M J
格式: Artigo
語言:Inglês
出版: 1989
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC210143/
https://ncbi.nlm.nih.gov/pubmed/2544563
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