In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon.

A mini-Mu bacteriophage containing a high copy number plasmid replicon was constructed to clone genes in vivo. A chloramphenicol resistance gene for independent selection and the lacZYA operon to form gene fusions were also incorporated into this phage. This mini-Mu element can transpose at a high f...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Groisman, E A, Castilho, B A, Casadaban, M J
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1984
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC344860/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6324195/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.5.1480
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