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Expression and purification of the p75 neurotrophin receptor transmembrane domain using a ketosteroid isomerase tag

BACKGROUND: Receptors with a single transmembrane (TM) domain are essential for the signal transduction across the cell membrane. NMR spectroscopy is a powerful tool to study structure of the single TM domain. The expression and purification of a TM domain in Escherichia coli (E.coli) is challenging...

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Bibliografiset tiedot
Päätekijät: Li, Qingxin, Chen, Angela Shuyi, Gayen, Shovanlal, Kang, CongBao
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: BioMed Central 2012
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3447675/
https://ncbi.nlm.nih.gov/pubmed/22510322
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-11-45
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