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Expression and purification of the p75 neurotrophin receptor transmembrane domain using a ketosteroid isomerase tag
BACKGROUND: Receptors with a single transmembrane (TM) domain are essential for the signal transduction across the cell membrane. NMR spectroscopy is a powerful tool to study structure of the single TM domain. The expression and purification of a TM domain in Escherichia coli (E.coli) is challenging...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
BioMed Central
2012
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3447675/ https://ncbi.nlm.nih.gov/pubmed/22510322 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-11-45 |
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