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Engineered Bi-Histidine Metal Chelation Sites Map the Structure of the Mechanical Unfolding Transition State of an Elastomeric Protein Domain GB1

Determining the structure of the transition state is critical for elucidating the mechanism behind how proteins fold and unfold. Due to its high free energy, however, the transition state generally cannot be trapped and studied directly using traditional structural biology methods. Thus, characteriz...

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Detalhes bibliográficos
Main Authors: Shen, Tao, Cao, Yi, Zhuang, Shulin, Li, Hongbin
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3443793/
https://ncbi.nlm.nih.gov/pubmed/22947942
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2012.07.019
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