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Dynamics of the l-fucose/H(+) symporter revealed by fluorescence spectroscopy
FucP of Escherichia coli catalyzes l-fucose/H(+) symport, and a crystal structure in an outward-facing conformation has been reported. However, nothing is known about FucP conformational dynamics. Here, we show that addition of l-fucose to purified FucP in detergent induces ∼20% quenching of Trp flu...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3443143/ https://ncbi.nlm.nih.gov/pubmed/22930818 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1213445109 |
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