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A fluorescence-detection size-exclusion chromatography-based thermostability assay to identify membrane protein expression and crystallization conditions

Optimization of membrane protein stability under different solution conditions is essential for obtaining crystals that diffract to high resolution. Traditional methods that evaluate protein stability require large amounts of material, and are therefore ill-suited for medium- to high-throughput scre...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
Main Authors: Hattori, Motoyuki, Hibbs, Ryan E., Gouaux, Eric
פורמט: Artigo
שפה:Inglês
יצא לאור: 2012
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC3441139/
https://ncbi.nlm.nih.gov/pubmed/22884106
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.str.2012.06.009
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