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A fluorescence-detection size-exclusion chromatography-based thermostability assay to identify membrane protein expression and crystallization conditions

Optimization of membrane protein stability under different solution conditions is essential for obtaining crystals that diffract to high resolution. Traditional methods that evaluate protein stability require large amounts of material, and are therefore ill-suited for medium- to high-throughput scre...

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Detalhes bibliográficos
Main Authors: Hattori, Motoyuki, Hibbs, Ryan E., Gouaux, Eric
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3441139/
https://ncbi.nlm.nih.gov/pubmed/22884106
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.str.2012.06.009
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