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Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins
GFP fusion-based fluorescence-detection size-exclusion chromatography (FSEC) has been widely employed for membrane protein expression screening. However, fused GFP itself may occasionally affect the expression and/or stability of the targeted membrane protein, leading to both false-positive and fals...
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| Publicado no: | Commun Biol |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7979870/ https://ncbi.nlm.nih.gov/pubmed/33742097 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s42003-021-01891-y |
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