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Separation of the complementary strands of DNA fragments on polyacrylamide gels.
32P-labeled (in vivo) phiX174 RFI DNA was restricted by Hinc II. Three aliquots of the same digest: a) nondenatured, b) heat denatured, and c) denatured by 5 mM Me-HgOH were analyzed on 3-15% acrylamide gel gradients or on 3% gels with reduced N,N'-methylene-bis-acrylamide. The autoradiography...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1977
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| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC343774/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/331258/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.5.1569 |
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