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Separation of the complementary strands of DNA fragments on polyacrylamide gels.

32P-labeled (in vivo) phiX174 RFI DNA was restricted by Hinc II. Three aliquots of the same digest: a) nondenatured, b) heat denatured, and c) denatured by 5 mM Me-HgOH were analyzed on 3-15% acrylamide gel gradients or on 3% gels with reduced N,N'-methylene-bis-acrylamide. The autoradiography...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Szalay, A A, Grohmann, K, Sinsheimer, R L
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1977
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC343774/
https://ncbi.nlm.nih.gov/pubmed/331258
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