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Separation of the complementary strands of DNA fragments on polyacrylamide gels.
32P-labeled (in vivo) phiX174 RFI DNA was restricted by Hinc II. Three aliquots of the same digest: a) nondenatured, b) heat denatured, and c) denatured by 5 mM Me-HgOH were analyzed on 3-15% acrylamide gel gradients or on 3% gels with reduced N,N'-methylene-bis-acrylamide. The autoradiography...
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| Auteurs principaux: | , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
1977
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| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC343774/ https://ncbi.nlm.nih.gov/pubmed/331258 |
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