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Utilization of an Escherichia coli mutant for carbon-13 enrichment of tRNA for NMR studies.
The enrichment of tRNA at specific sites with carbon-13 has been accomplished in vivo using a mutant of Escherichia coli. A relaxed strain of E. coli auxotrophic for methionine was grown in a specifically defined medium supplemented with either [14C] or [13C]-methyl labeled methionine. Cells were co...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1975
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC343518/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1101225/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/2.9.1503 |
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