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Utilization of an Escherichia coli mutant for carbon-13 enrichment of tRNA for NMR studies.

The enrichment of tRNA at specific sites with carbon-13 has been accomplished in vivo using a mutant of Escherichia coli. A relaxed strain of E. coli auxotrophic for methionine was grown in a specifically defined medium supplemented with either [14C] or [13C]-methyl labeled methionine. Cells were co...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Agris, P F, Fujiwara, F G, Schmidt, C F, Loeppky, R N
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 1975
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC343518/
https://ncbi.nlm.nih.gov/pubmed/1101225
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