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Utilization of an Escherichia coli mutant for carbon-13 enrichment of tRNA for NMR studies.
The enrichment of tRNA at specific sites with carbon-13 has been accomplished in vivo using a mutant of Escherichia coli. A relaxed strain of E. coli auxotrophic for methionine was grown in a specifically defined medium supplemented with either [14C] or [13C]-methyl labeled methionine. Cells were co...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
1975
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| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC343518/ https://ncbi.nlm.nih.gov/pubmed/1101225 |
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