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Hybrid isolation by recovery of RNA-DNA hybrids from agar using S(1) nuclease
A method for recovering RNA-DNA hybrids from agar employing a single strand specific nuclease is described. The procedure is suitable for large scale isolations, and immobilization of the DNA in agar prior to hybridization allows a high yield of hybrid without interference by DNA reannealing.
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1974
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC343380/ https://ncbi.nlm.nih.gov/pubmed/10793757 |
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