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Assay of DNA-RNA hybrids by S1 nuclease digestion and adsorption to DEAE-cellulose filters.
A fast and accurate assay procedure for DNA-RNA hybrids is described in which exhaustive digestion of unhybridized DNA with S1 nuclease is followed by binding of hybrids to filter discs of DEAE-cellulose. The digested DNA can be efficiently washed from the filters so that background levels of 0.1-0....
Gardado en:
| Publicado en: | Nucleic Acids Res |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
1978
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| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342142/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/673844/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.6.2033 |
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