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Assay of DNA-RNA hybrids by S1 nuclease digestion and adsorption to DEAE-cellulose filters.
A fast and accurate assay procedure for DNA-RNA hybrids is described in which exhaustive digestion of unhybridized DNA with S1 nuclease is followed by binding of hybrids to filter discs of DEAE-cellulose. The digested DNA can be efficiently washed from the filters so that background levels of 0.1-0....
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| Hlavní autoři: | , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
1978
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| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342142/ https://ncbi.nlm.nih.gov/pubmed/673844 |
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