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Hybrid isolation by recovery of RNA-DNA hybrids from agar using S(1) nuclease

A method for recovering RNA-DNA hybrids from agar employing a single strand specific nuclease is described. The procedure is suitable for large scale isolations, and immobilization of the DNA in agar prior to hybridization allows a high yield of hybrid without interference by DNA reannealing.

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Detalhes bibliográficos
Main Authors: Hansen, J.Norman, Pheiffer, Barbara H., Hough, Christopher J.
Formato: Artigo
Idioma:Inglês
Publicado em: 1974
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC343380/
https://ncbi.nlm.nih.gov/pubmed/10793757
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